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KMID : 0603820110170010069
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Journal of Experimental & Biomedical Science
2011 Volume.17 No. 1 p.69 ~ p.77
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Effect of Schizandra chinensis Extracts on Oxidative Damage
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Park Young-Mi
Lim Jae-Hwan
Jeong Hyung-Jin
Seo Eul-Won
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Abstract
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In this study, we evaluated the protective effects of supercritical extracts and two step ethanol extracts after supercritical extraction from Schizandra chinensis on antioxidant activities and oxidative DNA and cell damages. Supercritical extracts removed DPPH (1,1-diphenyl-2-picryldrazyl) radical by 85.5% at 200 μg/ml, but showed low activities of scavenging and chelating the hydroxyl radical and ferrous iron. However, two step ethanol extracts showed low activities of scavenging the DPPH radical, but removed the hydroxyl radical by 86% at 200 μg/ml. In addition, we tested the activities of extracts for reducing hydroxyl radical-induced DNA and cell damage. Two step ethanol extracts showed protective effect against the oxidative DNA damage by reducing DNA segmentation, inhibiting DNA migration and decreasing the expression of phospho-H2AX. Also, two step ethanol extracts showed protective effect against the oxidative cell damage by inhibiting lipid peroxidation and increasing the expression of p21 protein. Taken together, we suggest that two step ethanol extracts from S. chinensis have a role as useful inhibitors against oxidative damages.
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KEYWORD
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Schizandra chienesis, Hydroxyl radical, Antioxidant activity, Oxidative stress
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